Journal: The Journal of biological chemistry

Article Title: ATXN3 functions as a tumor suppresser through potentiating Galectin-9-mediated apoptosis in human colon adenocarcinoma.

doi: 10.1016/j.jbc.2024.107415

Figure Lengend Snippet: Figure 5. Targeted ATXN3 deletion inhibits colon cancer growth in mice. A–C, WT or ATXN3-KO HCT116 cells were injected subcutaneously into RAG1 mutant mice (n = 10). Tumor growth curve (A), photograph (B), and weight (C) are shown. D and E, the protein expression of Galectin-9, Ki67, Cleaved Caspase-3 in (B) tumor was detected by IHC and measured relative expression by image J software(n = 5). Scale bar: 200 mm. F–H, HCT116 cells were injected subcutaneously into RAG1 mutant mice and treated with Galectin-9 recombinant protein (n = 10). Tumor growth curve (F), photograph (G), and weight (H) are shown. I and J, the protein expression of Galectin-9, Ki67, Cleaved Caspase-3 in (G) tumor was detected by IHC and measured relative expression by image J software(n = 4). Scale bar: 200 mm. K–M, tumor growth curve (K) of RAG1 mutant mice injected subcutaneously with WT or ATXN3-KO HCT116 cells and stabilize overexpression of Galectin-9 by lentivirus (n = 20). Tumor photograph (L) and tumor weight (M) are shown. A, C, F, H, E, and J, 2- tailed unpaired t test. *p < 0.05, **p < 0.01, ***p < 0.001. L and M: ordinary 1-way ANOVA. *p < 0.05, **p < 0.01,***p < 0.001.

Article Snippet: In order to achieve in vivo overexpression of the recombinant protein Galectin-9, mice were administered intraperitoneal injections of Galectin-9 recombinant protein (R&D Systems) every 2 days, with an average dosage of 1 mg per injection.

Techniques: Injection, Mutagenesis, Expressing, Software, Recombinant, Over Expression

Journal: Cell metabolism

Article Title: Podoplanin-Expressing Macrophages Promote Lymphangiogenesis and Lymphoinvasion in Breast Cancer.

doi: 10.1016/j.cmet.2019.07.015

Figure Lengend Snippet: Figure 3. Perilymphatic Macrophage Localization Is Mediated by GAL8 Expression in LECs (A–C) Representative images of 4T1 tumor sections stained for GAL8 and PDPN (A), GAL8 and MECA-32 (B), and GAL8 and CD45 (C). (D and E) Quantification (D) and representative images (E) of WT or Pdpn KO BMDMs stained for GAL8 upon 40 min incubation with recombinant murine GAL8 (0.5 mM) or PBS (unstimulated).

Article Snippet: Prior to incubation with recombinant GAL8 (Novus Biologicals), cells were washed with PBS and incubated for 12 h in DMEMwithout FBS.

Techniques: Expressing, Staining, Incubation, Recombinant

Journal: Cell metabolism

Article Title: Podoplanin-Expressing Macrophages Promote Lymphangiogenesis and Lymphoinvasion in Breast Cancer.

doi: 10.1016/j.cmet.2019.07.015

Figure Lengend Snippet: Figure 5. PDPN-Mediated Adhesion of PoEMs to LECs Promotes Lymphatic Growth and Cancer Cell Lymphoinvasion (A and B) Quantification (A) and representative images (B) of WT or Pdpn KO BMDMs stained for activated form of integrin b1 (9EG7) upon 40 min treatment with recombinant murine GAL8 (0.5 mM) or normal medium (ctrl).

Article Snippet: Prior to incubation with recombinant GAL8 (Novus Biologicals), cells were washed with PBS and incubated for 12 h in DMEMwithout FBS.

Techniques: Staining, Recombinant

Journal: Cell metabolism

Article Title: Podoplanin-Expressing Macrophages Promote Lymphangiogenesis and Lymphoinvasion in Breast Cancer.

doi: 10.1016/j.cmet.2019.07.015

Figure Lengend Snippet: Figure 6. PDPN in PoEMs Is the Upstream Regulator of Matrix Remodeling, Independent of GAL8 Binding (A–M) qRT-PCR analysis of the expression of Mmps and collagen subunits in CD11b+, F4/80+ TAM populations sorted from 4T1 tumors: PoEMs (PDPN+), non- PoEMs (PDPN), the total WT TAM pool, and the total Pdpn KO TAM pool (PDPN).

Article Snippet: Prior to incubation with recombinant GAL8 (Novus Biologicals), cells were washed with PBS and incubated for 12 h in DMEMwithout FBS.

Techniques: Binding Assay, Quantitative RT-PCR, Expressing

Journal: Cell metabolism

Article Title: Podoplanin-Expressing Macrophages Promote Lymphangiogenesis and Lymphoinvasion in Breast Cancer.

doi: 10.1016/j.cmet.2019.07.015

Figure Lengend Snippet: Figure 7. Prospective Clinical Relevance of PoEMs in Breast Cancer Patients (A) FACS analysis of PDPN in human breast cancer macrophages (CD11b+, CD14+, and HLA-DR+), representing the fraction of PoEMs out of total TAMs. (B) Quantification of PDPN+ cells (IF) among CD11b+, CD14+, and HLA-DR+ TAMs (sorted from human breast tumor specimens), which have migrated through 8 mm pores (transwell) toward murine recombinant GAL8 (0.5 mM). (legend continued on next page) 14 Cell Metabolism 30, 1–20, November 5, 2019

Article Snippet: Prior to incubation with recombinant GAL8 (Novus Biologicals), cells were washed with PBS and incubated for 12 h in DMEMwithout FBS.

Techniques: Recombinant

Journal: Frontiers in Cellular Neuroscience

Article Title: Differential Cellular Expression of Galectin-1 and Galectin-3 After Intracerebral Hemorrhage

doi: 10.3389/fncel.2019.00157

Figure Lengend Snippet: Increased expression of Galectin-1 after ICH. (A) Confocal images depicting the temporal expression pattern of Galectin-1 after ICH (scale bar = 20 μm; n = 3–5 per group). (B) The average number of Galectin-1-positive cells per 0.1 mm 2 in the ipsilateral striatum. (C) Increased expression of Galectin-1 was confirmed by western blotting of brain tissue from the ipsilateral striatum. (D) Densitometry analysis ( n = 3–5 per group) of the western blotting data. ∗ p < 0.05, ∗∗∗ p < 0.001 vs. sham.

Article Snippet: Cells were then incubated with mouse recombinant Galectin-1 (6.25 or 12.5 μg/ml; R&D Systems, Minneapolis, MN, United States) for 1 h and it was followed by an 18-h treatment with LPS (100 ng/ml) or hemin (30 μg/ml).

Techniques: Expressing, Western Blot

Journal: Frontiers in Cellular Neuroscience

Article Title: Differential Cellular Expression of Galectin-1 and Galectin-3 After Intracerebral Hemorrhage

doi: 10.3389/fncel.2019.00157

Figure Lengend Snippet: Galectin-1 expression is mostly observed in glial cells after ICH. Brain sections were double immunostained for (A) Galectin-1 and GFAP, (B) Galectin-1 and Iba1, (the lowest panel depicts the high magnification images) and (C) Galectin-1 and NeuN (Scale bar = 20 μm; n = 3 per group). Galectin-1 expression was observed mostly in GFAP-positive cells and in a subset of Iba1-positive cells. NeuN positive cells didn’t express Galectin-1 ( n = 3 per group).

Article Snippet: Cells were then incubated with mouse recombinant Galectin-1 (6.25 or 12.5 μg/ml; R&D Systems, Minneapolis, MN, United States) for 1 h and it was followed by an 18-h treatment with LPS (100 ng/ml) or hemin (30 μg/ml).

Techniques: Expressing

Journal: Frontiers in Cellular Neuroscience

Article Title: Differential Cellular Expression of Galectin-1 and Galectin-3 After Intracerebral Hemorrhage

doi: 10.3389/fncel.2019.00157

Figure Lengend Snippet: Recombinant Galectin-1 and inflammatory response. Prior to LPS (100 ng/ml) stimulation, Raw 264.7 cells were treated with recombinant Galectin-1, and the release of IL-6 was measured using ELISA, as detailed in methods. Recombinant Galectin-1 significantly reduced LPS-induced release of IL-6 from RAW 246.7 cells ( n = 4 per group). ∗∗∗ p < 0.001 vs. LPS.

Article Snippet: Cells were then incubated with mouse recombinant Galectin-1 (6.25 or 12.5 μg/ml; R&D Systems, Minneapolis, MN, United States) for 1 h and it was followed by an 18-h treatment with LPS (100 ng/ml) or hemin (30 μg/ml).

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay

Journal: Nature medicine

Article Title: Injury-specific factors in the cerebrospinal fluid regulate astrocyte plasticity in the human brain.

doi: 10.1038/s41591-023-02644-6

Figure Lengend Snippet: Fig. 5 | CSF-CCM promotes proliferation and NSC response in hiPS cell- derived astrocytes by LGALS3BP. a, Schematic timeline of experimental paradigm. b, Representative micrographs showing immunostainings for the astrocyte marker S100B and FGFR3 in hiAstros. c, Disease-specific CSF effects on EdU-incorporation in hiAstros cell cultures. White arrowheads indicate examples of EdU+ hiAstros. d, The percentage of EdU+ hiAstros in different culture conditions. e,f, Representative images of immunostaining for EdU, GFAP/S100B and GAL3 of hiAstros in control (e) or CSF-containing cultures (f). Filled white arrowheads indicate GAL3+EdU+ cells; empty white arrowheads depict Gal3+EdU− astrocytes. g, Representative micrographs showing reduced hiAstros proliferation after exposure either to LGALS3BP-function blocking antibody MDP1959 alone or in combination with CSF-CCM. h,i, Phase-contrast images of primary neurospheres generated from hiAstros in control or CSF-

Article Snippet: CSF-CCM or CSF-ICM (100 μl ml−1 medium), MDP1959 (40 μl ml−1 medium) and/or recombinant human (rh) LGALS3BP (10 μg ml−1 or 20 μg ml−1 medium; R&D Systems 2226-GAB) were added at the beginning of the experiment only.

Techniques: Derivative Assay, Marker, Immunostaining, Control, Blocking Assay, Generated